Human Serpin D1/Heparin Cofactor II Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3198
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Neutralization, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin D1/Heparin Cofactor II
Gly20-Ser499
Accession # P05546
Gly20-Ser499
Accession # P05546
Specificity
Detects human Serpin D1/Heparin Cofactor II in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse Serpin D1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Serpin D1/Heparin Cofactor II Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin D1/
Heparin Cofactor II (Catalog # 3198-PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin D1/
Heparin Cofactor II (Catalog # 3198-PI), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Serpin D1/Heparin Cofactor II (Catalog # 3198-PI)
Sample: Recombinant Human Serpin D1/Heparin Cofactor II (Catalog # 3198-PI)
Neutralization
Measured by its ability to neutralize Recombinant Human Serpin D1 (0.56 µg/mL, Catalog # 3198-PI) inhibition of Recombinant Human Coagulation Factor II/Thrombin (0.05 µg/mL, Catalog # 1473-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (100 µM, Catalog # ES011). The Neutralization Dose (ND50) is typically 6.6 µg/mL.
Reviewed Applications
Read 1 review rated 4 using AF3198 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin D1/Heparin Cofactor II
Serpin D1, also known as Heparin Cofactor II, is a member of the Serpin superfamily of the serine protease inhibitors (1). Similar to Serpins A5 and C1, it inhibits thrombin and this activity is enhanced by heparin. Interestingly, a C-terminal His-tagged recombinant Serpin D1 had enhanced heparin effect, which was maintained in a plasma-based thrombin inhibition assay (2). Congenital Serpin D1 deficiency is a potential risk factor for thrombosis (3). The full-length human cDNA was expressed and the purified protein corresponded to the secreted form with anti-thrombin activity.
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Bauman, S.J. et al. (1999) J. Biol. Chem. 274:34556.
- Tollefsen, D.M. (2002) Arch. Pathol. Lab. Med. 126:1394.
Alternate Names
HC2, HCF2, Heparin Cofactor II, LS2
Gene Symbol
SERPIND1
UniProt
Additional Serpin D1/Heparin Cofactor II Products
Product Specific Notices for Human Serpin D1/Heparin Cofactor II Antibody
For research use only
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