Detects human Serpin A7/TBG in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Serpin A9 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Serpin A7/TBG Antibody

Detection of Human Serpin A7/TBG by Western Blot.

Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Serpin A7/TBG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8176) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Serpin A7/TBG at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Serpin A7/TBG Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Human plasma

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A7/TBG

Serpin A7 (also TBG/Thyroxine-binding globulin and T4-binding globulin) is a secreted 54 kDa glycoprotein, non-inhibitory member of the serpin family of proteins. It is expressed by the liver and secreted in plasma. Mutations in the SERPINA7 gene can result in a whole spectrum of deficiencies, characterized by either reduced or increased TBG levels in the serum. A deficiency in TBG result in low serum total T4 and T3 count in clinically euthyroid subjects in the presence of normal serum TSH. Over aa21-415, human serpin A7 shares 76% aa sequence identity with mouse serpin A7.

Alternate Names

TBG

Entrez Gene IDs

6906 (Human); 331535 (Mouse); 81806 (Rat)

Gene Symbol

SERPINA7

UniProt

P05543

Additional Serpin A7/TBG Products

Product Documents for Human Serpin A7/TBG Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human Serpin A7/TBG Antibody

For research use only

Citations
Reviews

Human Serpin A7/TBG Antibody

R&D Systems, part of Bio-Techne | Catalog # AF8176

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity