Detects human ROBO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant rat ROBO1, recombinant human (rh) ROBO3 and rhROBO4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human ROBO2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: NTera-2 human testicular embryonic carcinoma cell line differentiated with retinoic acid

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human neural progenitor cells differentiated by growth factor withdrawal

Western Blot

0.1 µg/mL
Sample: Recombinant Human ROBO2 Fc Chimera (Catalog # 3147-RB)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROBO2

Human ROBO2 is a 175 kDa member of the ROBO/roundabout receptor family and is a 1357 amino acid (aa) type I transmembrane (TM) protein. It contains an extracellular domain (ECD) comprised of five C2-type Ig-like domains and three fibronectin type III domains. ROBO receptors interact with SLIT proteins to regulate axon guidance. Similar to ROBO1, the first two Ig-like domains of ROBO2 are predicted to be important for SLIT binding. Alternate splice forms of ROBO2 exist. The ROBO2 ECD in human shares 98% aa identity with the ROBO2 ECD in mouse and canine.

Long Name

Roundabout Homolog 2

Alternate Names

SAX3

Entrez Gene IDs

6092 (Human); 268902 (Mouse); 84409 (Rat)

Gene Symbol

ROBO2

UniProt

Q9HCK4

Additional ROBO2 Products

Product Documents for Human ROBO2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ROBO2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ROBO2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3147

Key Product Details

Species Reactivity

Validated:

Cited:

Applications