Western blot shows lysates of human brain (cerebellum) tissue and human brain (hypothalamus) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Rit2 Monoclonal Antibody (Catalog # MAB6094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Rit2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Rit2 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion-fixed paraffin-embedded sections of human brain (caudate putamen)

Western Blot

2 µg/mL
Sample: Human brain (cerebellum) tissue and human brain (hypothalamus) tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rit2

GTP-binding protein Rit2 (Rit2; also Rin, Ras-like protein expressed in neurons, Ras-like without CAAX) is a 25 kDa member of the small GTPase superfamily and Ras family. Human Rit2 is 217 amino acids (aa) in length. Like all small G-proteins, Rit2 contains five highly conserved domains (G1-G5) and acts as a molecular switch by alternating an active GTP-bound form and an inactive GDP-bound form. There are two isoforms for Rit2. Isoform 1 is the long form. Isoform 2 has an 11 aa substitution for residues 143‑153 found in isoform 1 and a deletion of aa 154‑217. Rit2 is expressed exclusively in neurons. In addition, Rit2 binds calmodulin through a C‑terminal binding motif.

Long Name

Ras-like without CAAX 2

Alternate Names

RIBA, RIN, ROC2

Entrez Gene IDs

6014 (Human); 19762 (Mouse); 291713 (Rat)

Gene Symbol

RIT2

UniProt

Q99578 (Human)

Additional Rit2 Products

Product Documents for Human Rit2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Rit2 Antibody

For research use only

Citations
Reviews

Human Rit2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6094

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Rit2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Rit2 Antibody

Detection of Human Rit2 by Western Blot.