Human RIG-I Alexa Fluor™ Plus 680-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4859AFP680
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human RIG-I
Specificity
Detects human RIG-I in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human RIG-I Alexa Fluor™ Plus 680-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: RIG-I
RIG-I (Retinoic-acid inducible gene I; also Dead-box protein 58) is a 100 kDa member of the DexH-box family of proteins. It is expressed in smooth muscle cells and endothelium. RIG-I is normally an inactive monomer. Upon Sendai and Hepatitis C virus infection, RIG-I binds to dsRNA, initiating homodimerization and downstream activation of IRF3 and KF kappaB genes. Human RIG-I is 925 amino acids (aa) in length. It contains two CARD domains (aa 1‑172), one helicase ATP-binding region (aa 251‑430), and one C-terminal repressor domain (aa 735‑925). There are three potential splice variants. One shows an alternate start site at Met204, while another shows deletion of aa 3‑80, and a third shows a deletion of aa 36‑80 plus 749‑925. Over aa 724‑925, human RIG-I is 76% aa identical to mouse RIG-I.
Long Name
RIG-I
Alternate Names
DDX58, RIGI
Gene Symbol
DDX58
UniProt
Additional RIG-I Products
Product Specific Notices for Human RIG-I Alexa Fluor™ Plus 680-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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