Western blot shows lysates of HEK293 human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Rictor Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4598) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Rictor at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Rictor Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rictor

Rictor (rapamycin-insensitive companion of mTOR) is a member of the mTORC2 complex, which regulates cell growth by integrating stress, energy, and nutrient signals with growth factor derived signals. Rictor contains no obvious catalytic motifs, but knockdown studies of mTOR or Rictor results in a loss actin polymerization and cell spreading. In vitro, the mTOR-Rictor complex phosphorylates Akt/PKB on Ser473, an essential step for full activation of Akt/PKB. Additionally, recent studies have shown that mTOR-Rictor regulates PKC alpha phosphorylation and PDGFR beta expression and signaling.

Long Name

Rapamycin-insensitive Companion of mTOR

Alternate Names

mAVO3, Pianissimo

Entrez Gene IDs

253260 (Human)

Gene Symbol

RICTOR

UniProt

Q6R327

Additional Rictor Products

Product Documents for Human Rictor Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Rictor Antibody

For research use only

Citations
Reviews

Human Rictor Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4598

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Rictor Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Rictor Antibody

Detection of Human Rictor by Western Blot.