Human RBPMS Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11670
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1090828
Product Specifications
Immunogen
Synthetic Peptide
Accession # Q93062
Accession # Q93062
Specificity
Detects a peptide specific for human RBPMS around amino acid 150 in Direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human RBPMS Antibody
Detection of Human RBPMS by Western Blot.
Western Blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human RBPMS Monoclonal Antibody (Catalog # MAB11670) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for RBPMS at approximately 28, 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of RBPMS in Human Testis.
RBPMS was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human RBPMS Monoclonal Antibody (Catalog # MAB11670) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm of smooth muscle. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human RBPMS Antibody
Application
Recommended Usage
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis
Sample: Immersion fixed paraffin-embedded sections of human testis
Western Blot
2 µg/mL
Sample: A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line and JEG-3 human epithelial choriocarcinoma cell line
Sample: A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line and JEG-3 human epithelial choriocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RBPMS
References
- Nakagaki-Silva EE, Gooding C, Llorian M, Jacob AG, Richards F, Buckroyd A, Sinha S, Smith CWJ. Identification of RBPMS as a mammalian smooth muscle master splicing regulator via proximity of its gene with super-enhancers. Elife. 2019 Jul 8;8:e46327. doi: 10.7554/eLife.46327. PMID: 31283468; PMCID: PMC6613909.
- Rabelo-Fernández RJ, Santiago-Sánchez GS, Sharma RK, Roche-Lima A, Carrion KC, Rivera RAN, Quiñones-Díaz BI, Rajasekaran S, Siddiqui J, Miles W, Rivera YS, Valiyeva F, Vivas-Mejia PE. Reduced RBPMS Levels Promote Cell Proliferation and Decrease Cisplatin Sensitivity in Ovarian Cancer Cells. Int J Mol Sci. 2022 Jan 4;23(1):535. doi: 10.3390/ijms23010535. PMID: 35008958; PMCID: PMC8745614.
Long Name
RNA binding protein, mRNA processing factor
Alternate Names
HERMES, RBPMS
Gene Symbol
RBPMS
UniProt
Additional RBPMS Products
Product Documents for Human RBPMS Antibody
Product Specific Notices for Human RBPMS Antibody
For research use only
Loading...
Loading...
Loading...
Loading...