Human/Rat PAK7 Antibody

Detection of PAK7 by Western Blot

PAK5 & AIF expression positively correlated with poor prognosis of breast cancer. (a) Analysis of expression differential proteins regulated by PAK5. Lysates from MDA-MB-231 overexpressing Flag-vector or Flag-tagged PAK5 & screening of differentially expressed proteins by sequencing. (b) Predict the expression of AIF in breast cancer & adjacent cancer in TCGA database. The fold-change was 0.897. (c) Predict the overall survival of AIF in GSE31519 database. (d) Predict the overall survival of AIF in TCGA database. (e) PAK5 & AIF expression in 122 clinical breast tissue pairs. Lysates of tumor tissues (T) & matched adjacent noncancerous tissues (N) were analyzed using Western blotting. Twelve representative pairs are shown. (f) The indicated protein levels in (e) were statistically analyzed (****p < 0.001). (g) Spearman's rank test was used to analyze the correlation between AIF relative expression & PAK5 relative expression in 122 subjects. (h) Representative images of immunohistochemical staining showing PAK5 & AIF protein expression in breast cancer. Original magnification, 400×. IHC staining was scored, & a Pearson correlation test was performed. Note that the scores of some samples overlap. (i-l) Kaplan-Meier survival analysis (GraphPad) of the relationship between overall survival (upper) & disease-free survival (lower) in breast cancer cases & PAK5 and/or AIF expression. The subjects were divided into different groups based on indicated PAK5 & AIF expression scores in the tumors: PAK5 low (n = 35) & PAK5 high (n = 40, i); AIF low (n = 36) & AIF high (n = 39, j). n = 15 for PAK5 low/AIF low, n=4 for PAK5 high/AIF low, n=12 for PAK5 low/AIF high, n = 44 for PAK5 high/AIF high; n = 35 for high nuclear translocation ratio of AIF, n = 40 for low nuclear translocation ratio of AIF. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33867848), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of PAK7 by Western Blot

PAK5 inhibits AIF release by regulating mitochondrial membrane permeability and potential. (a) PAK5 could increase the co-localization of AIF and COXIV (mitochondrial protein). Nucleus was stained with DAPI (4', 6 diamidino-2-phenylindole). Yellow indicates co-localization. Original magnification, × 600. The Pearson's correlation and overlap co-efficient were shown in bar graph format (30 cells) from three independent experiments were analyzed (error bars, SEM). (b,c) PAK5 could increase the expression of AIF in mitochondria. Cells were stably transfected with Flag-tagged PAK5 and extracted mitochondrial protein. Protein expression levels were determined by Western blotting. The data are shown as the mean _ SEM of triplicate experiments (*p < 0.05, **P < 0.01 vs. Control, n =3). (d-g) PAK5 could reduce membrane permeability of mitochondria. Cells were stably transfected with Flag-tagged PAK5 (d,e) or infected with PAK5-RNAi lentivirus (f,g) and extracted mitochondrial protein. Protein expression levels were determined by Western blotting. The data are shown as the mean _ SEM of triplicate experiments (***p < 0.001, ****p < 0.0001 vs. Control, n =3). (h-k) PAK5 can increase the membrane potential of mitochondria. Cells were stably transfected with Flag-tagged PAK5 (h,j) or infected with PAK5-RNAi lentivirus (i,k), the change in deltaΨm was examined using JC-1 staining assay. The ratio of fluorescent intensity of J-aggregates and monomers in treated cells is shown in e and f. The data are shown as the mean _ SEM of triplicate experiments (**P < 0.01, ***p < 0.001 vs. Control, n =3). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33867848), licensed under a CC-BY license. Not internally tested by R&D Systems.