Detects endogenous human and rat CaMKK alpha in Western blots. In Western blots, this antibody does not cross-react with recombinant human CaMKK beta.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Rat CaMKK alpha Antibody

Detection of Human/Rat CaMKK alpha by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CCD-1070Sk human foreskin fibroblast cell line, K562 human chronic myelogenous leukemia cell line, and human brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Rat CaMKKa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4536) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CaMKK alpha at approximately 68 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Detection of Human CaMKK alpha by Simple Western^TM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CaMKKa at approximately 68 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Rat CaMKKa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4536) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Rat CaMKK alpha Antibody

Application

Recommended Usage

Simple Western

10 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, CCD-1070Sk human foreskin fibroblast cell line, K562 human chronic myelogenous leukemia cell line, and human brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CaMKK alpha

The calcium/calmodulin (CaM) kinase signaling cascade includes the upstream kinases CaM kinase kinase alpha (CaMKK alpha; also known as CaMKK1) and CaMKK beta (CaMKK2), and their substrates, the output kinases CaMKI and CaMKIV. Largely by activation of the transcription factor CREB, the CaM kinase cascade is implicated in neuronal development and long-term memory formation. In addition, CaMKK alpha is known to modulate skeletal muscle glucose uptake, and is upregulated during retinoic acid-induced neutrophil maturation.

Long Name

Calcium/Calmodulin-dependent Protein Kinase Kinase alpha

Alternate Names

CaM-kinase IV kinase, CaMKK1

Entrez Gene IDs

84254 (Human); 55984 (Mouse); 60341 (Rat)

Gene Symbol

CAMKK1

UniProt

Q8N5S9

Additional CaMKK alpha Products

Product Documents for Human/Rat CaMKK alpha Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Rat CaMKK alpha Antibody

For research use only

Citations
Reviews

Human/Rat CaMKK alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4536

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity