Western blot shows human plasma. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Protein S/PROS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4036) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). Specific bands were detected for Protein S/PROS1 at approximately 80 & 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Protein S/PROS1 by Simple Western^TM.

Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Protein S/PROS1 at approximately 106 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Protein S/PROS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4036) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Protein S/PROS1 Antibody

Application

Recommended Usage

Simple Western

2 µg/mL
Sample: Human plasma

Western Blot

0.2 µg/mL
Sample: Human plasma

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protein S/PROS1

Protein S is a 69 kDa, secreted glycoprotein that belongs to a general group of vitamin K-dependent proteins. It is synthesized principally by hepatocytes, circulates both free (40%) and bound to C4bBP (60%), interacts with protein C to suppress coagulation, and binds TAM family RTKs during immune regulation. Mature human Protein S is 634 amino acids (aa) in length. It contains one Gla domain (aa 42‑87), four EGF-like repeats (aa 117-283) and two laminin G-like domains (aa 299‑666). Over aa 42-670, human Protein S shares 83%, 80%, and 79% aa sequence identity with porcine, mouse, and canine Protein S, respectively.

Alternate Names

PROS1

Entrez Gene IDs

5627 (Human); 19128 (Mouse); 81750 (Rat)

Gene Symbol

PROS1

UniProt

P07225

Additional Protein S/PROS1 Products

Product Documents for Human Protein S/PROS1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Protein S/PROS1 Antibody

For research use only

Citations
Reviews

Human Protein S/PROS1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4036

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Protein S/PROS1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Protein S/PROS1 Antibody

Detection of Human Protein S/PROS1 by Western Blot.

Detection of Human Protein S/PROS1 by Simple WesternTM.