Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Protein O-Glucosyltransferase 1/POGLUT Monoclonal Antibody (Catalog # MAB6437) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Protein O-Glucosyltransferase 1/POGLUT at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: JEG‑3 human epithelial choriocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protein O-Glucosyltransferase 1/POGLUT1

O-Glucosyltransferase1 (POGLUT1) is a homologue of Rumi from Drosophila, an endoplasmic reticulum (ER)-retaining glucosyltransferase, that adds glucose to serine residues within the consensus sequence of C1‑X‑S‑X‑P‑C2 in Notch EGF repeats, thereby regulating cell-fate decisions (1). It is also known as CAP10-like protein (2) and KTELC1 due to the highly conserved CAP10 domain and the presence of an ER-retaining motif, KTEL, at the C‑terminus. The human gene is reported to be involved in the pathogenesis of both acute myelogenous and T‑acute lymphoblastic leukemias (3). POGLUT1 has hydrolase activity on UDP-Glc in a phosphatase-coupled glycosyltransferase assay (4).

References

Acar, M. et al. (2008) Cell 132:247.
Teng, Y. et al. (2006) Gene 371:7
Wang, Y. et al. (2010) Genet Test Mol Biomarkers 14:127.
Wu, Z.L. et al. (2010) Glycobiology, in press.

Alternate Names

CLP46, KDELCL1, KTELC1, MDS010, MDSRP, POGLUT1, Rumi

Entrez Gene IDs

56983 (Human)

Gene Symbol

POGLUT1

UniProt

Q8NBL1

Additional Protein O-Glucosyltransferase 1/POGLUT1 Products

Product Documents for Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody

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COA

SDS

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Product Specific Notices for Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody

For research use only

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Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6437

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody

Detection of Human Protein O‑Glucosyltransferase 1/POGLUT by Western Blot.