Human Prostaglandin E Synthase 2/
PTGES2 Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7627U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Prostaglandin E Synthase 2/PTGES2
Glu88-His377
Accession # Q9H7Z7
Glu88-His377
Accession # Q9H7Z7
Specificity
Detects human Prostaglandin E Synthase 2/PTGES2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Prostaglandin E Synthase 2/PTGES2
PTGES2 (Prostaglandin E Synthase 2; also C9orf15, GBF-1 and mPGES2) is a 32 kDa member of the GST superfamily of molecules. It is a constitutively expressed, integral membrane protein embedded in the Golgi apparatus, and is found in select cell types, including striated muscle cells, neurons, hepatocytes and astrocytes and endothalium. PTGES2 is proposed to lie at the end of a PGE2 synthetic pathway. PLA2S is known to first releases arachidonic acid (AA) from membrane phospholipids. This AA is next converted to PGH2 by COX-1/-2, and the PGH2 is then potentially isomerized into PGE2 by PTGES type enzymes. Notably, PTGES2 is not a glutathione-dependent enzyme, and some evidence suggests it is not a functional prostaglandin synthase. Human PTGES2 is potentially a 377 amino acid (aa) type III (no signal sequence) transmembrane protein. It contains a 57 aa luminal region, a 17 aa transmembrane segment (aa 58-74) and a 303 aa cytoplasmic domain (aa 75-377). There is one glutaredoxin domain (aa 90-193) and a GST-like region (aa 263-377). Proteolytic cleavage between Ala87Glu88 of 42-43 kDa full-length PTGES2 generates a soluble 32 kDa short form that localizes perinuclearly. There are two potential alternative splice forms. One contains a 19 aa insertion after Ser159, while a second utilizes an alternative start site at Met192. Over aa 88-377, human PTGES2 shares 91% aa sequence identity with mouse PTGES2.
Alternate Names
C9orf15, GBF1, mPGES2, PGES2
Gene Symbol
PTGES2
UniProt
Additional Prostaglandin E Synthase 2/PTGES2 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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