Human/Primate MMP-9 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF911
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Primate
Cited:
Human
Applications
Validated:
Western Blot, ELISA Detection (Matched Antibody Pair)
Cited:
ELISA Detection, ELISA Development (Detection), ELISA Microarray Development, Luminex Development
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Specificity
Detects human and primate MMP-9 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with rmMMP-9, rhMMP-1, -2, -3, -7, -8, -10, -12, -13, and -14 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Primate MMP-9 Biotinylated Antibody
Human MMP-9 ELISA Standard Curve
Recombinant Human MMP-9 (Catalog # 911-MP) was serially diluted and captured by Mouse Anti-Human/Primate MMP-9 Monoclonal Antibody (Catalog # MAB936) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human/Primate MMP-9 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human MMP‑9 (Catalog # 911-MP)
Sample: Recombinant Human MMP‑9 (Catalog # 911-MP)
Human/Primate MMP-9 Sandwich Immunoassay
ELISA Detection (Matched Antibody Pair)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-9
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.
Long Name
Matrix Metalloproteinase 9
Alternate Names
CLG4B, Gelatinase B, GELB, MANDP2, MMP9
Gene Symbol
MMP9
Additional MMP-9 Products
Product Documents for Human/Primate MMP-9 Biotinylated Antibody
Product Specific Notices for Human/Primate MMP-9 Biotinylated Antibody
For research use only
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