PPARa/NR1C1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Rabbit Anti-Human PPARa/NR1C1 Monoclonal Antibody (Catalog # MAB24781) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human PPAR alpha/NR1C1 Antibody

Application

Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HT-29 human colon adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PPAR alpha/NR1C1

Peroxisome proliferator activated receptor alpha (PPARA) a member of the steroid hormone receptor superfamily, is a nuclear transcription factor which mediates the activity of peroxisome proliferators. PPAR alpha exhibits the highest affinity with unsaturated fatty acids, and linolenic acids. PPAR alpha is expressed in brown fat, liver, kidney, heart, mucosa of the stomach and duodenum, retina, adrenal gland, skeletal muscle, pancreatic islets and smooth muscle cells. PPAR alpha plays important roles in lipid and glucose metabolism, and has been implicated in obesity-related metabolic diseases such as hyperlipidemia, insulin resistance, and coronary artery disease.

Long Name

Peroxisome Proliferator-activated Receptor alpha

Alternate Names

NR1C1, PPARA

Entrez Gene IDs

5465 (Human)

Gene Symbol

PPARA

UniProt

Q07869

Additional PPAR alpha/NR1C1 Products

Product Documents for Human PPAR alpha/NR1C1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human PPAR alpha/NR1C1 Antibody

For research use only

Citations
Reviews

Human PPAR alpha/NR1C1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB24781

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all PPAR alpha/NR1C1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human PPAR alpha/NR1C1 Antibody

PPAR alpha/NR1C1 in HT-29 Human Cell Line.