POU2F3 was detected in immersion fixed paraffin-embedded sections of human melanoma using Mouse Anti-Human POU2F3 Monoclonal Antibody (Catalog # MAB11625) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human POU2F3 Antibody

Application

Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human melanoma

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: POU2F3

POU2F3 is a 47 kDa transcription factor involved in the regulation of the differentiation of keratinocytes. It is also a part of the POU2F3-POU2AF2/POU2AF3 complex that drives the expression of tuft-cell-specific genes. Normal and neoplastic tuft cells share a genetic requirement for POU2F3. POU2F3 is utilized as an ancillary marker for the diagnosis of neuroendocrine-low small cell lung cancer. It is also positive in mesenchymal and neuroectodermal tumors including synovial carcinoma, solitary fibrous tumor, glioblastoma, Wilms tumor and melanoma.

References

Hildesheim J, Kühn U, Yee CL, Foster RA, Yancey KB, Vogel JC. The hSkn-1a POU transcription factor enhances epidermal stratification by promoting keratinocyte proliferation. J Cell Sci. 2001 May;114(Pt 10):1913-23. doi: 10.1242/jcs.114.10.1913. PMID: 11329378.
Wu XS, He XY, Ipsaro JJ, Huang YH, Preall JB, Ng D, Shue YT, Sage J, Egeblad M, Joshua-Tor L, Vakoc CR. OCA-T1 and OCA-T2 are coactivators of POU2F3 in the tuft cell lineage. Nature. 2022 Jul;607(7917):169-175. doi: 10.1038/s41586-022-04842-7. Epub 2022 May 16. PMID: 35576971; PMCID: PMC9419707.
Kaczorowski M, Ylaya K, Chłopek M, Lasota J, Miettinen M. Expression of POU2F3 Transcription Factor and POU2AF2, POU2F3 Coactivator, in Tuft Cell-like Carcinoma and Other Tumors. Am J Surg Pathol. 2024 Sep 25. doi: 10.1097/PAS.0000000000002313. Epub ahead of print. PMID: 39319626.

Long Name

POU class 2 homeobox 3

Alternate Names

Epoc-1, OCT-11, OCT11, OTF-11, PLA-1, PLA1, Skn-1a

Entrez Gene IDs

25833 (Human); 18988 (Mouse); 116544 (Rat)

Gene Symbol

POU2F3

UniProt

Q9UKI9 (Mouse)

Additional POU2F3 Products

Product Documents for Human POU2F3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human POU2F3 Antibody

For research use only

Citations
Reviews

Human POU2F3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11625

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all POU2F3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human POU2F3 Antibody

Detection of POU2F3 in Human Melanoma.