Detects human Plexin A1 in direct ELISAs. In Western blots, no cross-reactivity with recombinant human Plexin A4, B1, B2, B3, C1, D1, recombinant mouse Plexin A1, A2, or A3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Plexin A1 Antibody

Detection of Human Plexin A1 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human Plexin A1 Monoclonal Antibody (Catalog # MAB6536) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Plexin A1 at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Plexin A1 in Human Dendritic Cells.

Plexin A1 was detected in immersion fixed immature human dendritic cells using Mouse Anti-Human Plexin A1 Monoclonal Antibody (Catalog # MAB6536) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Plexin A1 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed immature human dendritic cells

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin A1

Plexin A1 is an approximately 200 kDa type I transmembrane member of the Plexin family of Semaphorin signal transducers. It consists of a 1218 amino acid (aa) extracellular domain with one Sema domain, a spacer, and four tandem IPT/TIG domains, a 21 aa transmembrane segment, and a 631 aa cytoplasmic domain. Plexin A1 binds the transmembrane Semaphorin 6D directly and binds the Class 3 secreted Semaphorins indirectly via Neuropilin-1 and -2. It mediates cytoskeletal remodeling that results in cell migration and axon repulsion. Within aa 964-1152 (in the IPT/TIG region), human Plexin A1 shares 93% aa sequence identity with mouse and rat Plexin A1.

Alternate Names

NOVP, PLXN1, PLXNA1

Entrez Gene IDs

5361 (Human); 18844 (Mouse)

Gene Symbol

PLXNA1

UniProt

Q9UIW2

Additional Plexin A1 Products

Product Documents for Human Plexin A1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Plexin A1 Antibody

For research use only

Citations
Reviews

Human Plexin A1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6536

Key Product Details

Species Reactivity

Validated:

Cited:

Applications