Detects human PLA2G1B in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) PLA2G2A, rhPLA2G7, recombinant mouse (rm) PLA2G2A, or rmPLA2R1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human PLA2G1B Antibody

Detection of Human PLA2G1B by Western Blot.

Western blot shows lysates of human pancreas tissue. PVDF Membrane was probed with 2 µg/mL of Human PLA2G1B Monoclonal Antibody (Catalog # MAB5018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLA2G1B at approximately 17 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PLA2G1B Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human PLA2G1B (Catalog # 5018‑PL)

Western Blot

2 µg/mL
Sample: Human pancreas tissue under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLA2G1B

PLA2G1B, also called Secretory Phopholipase A2, hydrolyses the sn-2 ester bond of phospholipids, including those in cell membranes, to generate non-esterified free fatty acids and lysophospholipids. Most secretory PLA2s are stored in cytoplasmic granules and are released into plasma and biological fluids on appropriate cell activation, especially during systemic inflammatory, autoimmune, or allergic disease. PLA2G1B is abundant in digestive organs but is also found elsewhere. Human PLA2G1B shares 78% and 76% amino acid sequence identity with mouse and rat PLA2G1B, respectively.

Long Name

Phospholipase A2 Group IB

Alternate Names

PLA2, PLA2A, PPLA2

Entrez Gene IDs

5319 (Human); 18778 (Mouse); 29526 (Rat)

Gene Symbol

PLA2G1B

UniProt

NM_000928 (Human)

Additional PLA2G1B Products

Product Documents for Human PLA2G1B Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human PLA2G1B Antibody

For research use only

Citations
Reviews

Human PLA2G1B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5018

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all PLA2G1B Antibodies »

Product Specifications

Immunogen

Specificity