Human Phospho-Src (Y419) Alexa Fluor® 647-conjugated Antibody

Name: Human Phospho-Src (Y419) Alexa Fluor® 647-conjugated Antibody
Brand: R&D Systems
SKU: AF2685R

R&D Systems, part of Bio-Techne | Catalog # AF2685R

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Product Datasheet / COA

Key Product Details

Species Reactivity:

Human

Immunogen:

Phosphopeptide containing human Src Y419 site

Applications:

Immunohistochemistry, Western Blot

Label:

Alexa Fluor 647 (Excitation=650 nm, Emission=668 nm)

Specificity:

Detects human Src family members when phosphorylated at sites corresponding to Y419 of human Src in Western blots.

View Full Technical Details for Human Phospho-Src (Y419) Alexa Fluor® 647-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: Src

Src and other Src family protein tyrosine kinases are proto-oncogenes that play key roles in regulating cell growth and differentiation. Src family activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation at Y419 of human Src up-regulates kinase activity, while phosphorylation at Y530 inactivates Src.

Item	Value
Long Name	v-src Sarcoma [Schmidt-Ruppin A-2] Viral Oncogene Homolog
Entrez Gene IDs	6714 (Human); 20779 (Mouse); 83805 (Rat); 1491925 (Viral)
Alternate Names	ASV, EC 2.7.10, EC 2.7.10.2, RSVgp4, Rous sarcoma, Src, c-Src, pp60c-src, tyrosine kinase pp60c-src, tyrosine-protein kinase SRC-1, v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog, v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

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