Western blot shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 30 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Monoclonal Antibody (Catalog # MAB54361) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-Ribosomal Protein S6/RPS6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospho-Ribosomal Protein S6/RPS6 (S235/S236) in MCF‑7 Human Cell Line.

Ribosomal Protein S6/RPS6 was detected in immersion fixed MCF-7 human breast cancer cell line untreated or treated with Rapamycin using Rat Anti-Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Monoclonal Antibody (Catalog # MAB54361) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Antibody

Application

Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Western Blot

1 µg/mL
Sample: HEK293T human embryonic kidney cell line treated with Recombinant Human EGF (Catalog # 236-EG)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ribosomal Protein S6/RPS6

40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens. RPS6 is dephosphorylated during growth arrest.

Alternate Names

RPS6

Entrez Gene IDs

6194 (Human); 20104 (Mouse); 29304 (Rat)

Gene Symbol

RPS6

UniProt

P62753

Additional Ribosomal Protein S6/RPS6 Products

Product Documents for Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Antibody

For research use only

Citations
Reviews

Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB54361

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Ribosomal Protein S6/RPS6 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Antibody

Detection of Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) by Western Blot.