Human Peroxiredoxin 1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3488
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Knockout Validated, Western Blot, Immunocytochemistry, Simple Western, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 477609
Product Specifications
Immunogen
E. coli-derived recombinant human Peroxiredoxin 1
Met1-Lys199
Accession # Q06830
Met1-Lys199
Accession # Q06830
Specificity
Detects endogenous human Peroxiredoxin 1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Peroxiredoxin 1 Antibody
Detection of Human Peroxiredoxin 1 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Human Peroxiredoxin 1 Monoclonal Antibody (Catalog # MAB3488) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Peroxiredoxin 1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Peroxiredoxin 1 in MCF-7 Human Cell Line.
Peroxiredoxin 1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Peroxiredoxin 1 Monoclonal Antibody (Catalog # MAB3488) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human Peroxiredoxin 1 by Simple WesternTM.
Simple Western shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Peroxiredoxin 1 at approximately 28 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Peroxiredoxin 1 Monoclonal Antibody (Catalog # MAB3488). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.Applications for Human Peroxiredoxin 1 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Immunoprecipitation
1 µg/1 mg cell lysate
Sample: Cell lysate of U2OS human osteosarcoma cell line
Sample: Cell lysate of U2OS human osteosarcoma cell line
Knockout Validated
Peroxiredoxin 1 is specifically detected in the parental U2OS cell line, but is not detectable in knockout U2OS cell line.
Simple Western
10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Western Blot
0.1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and MCF-7 human breast cancer cell line
Sample: Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and MCF-7 human breast cancer cell line
Reviewed Applications
Read 1 review rated 5 using MAB3488 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peroxiredoxin 1
Alternate Names
NKEF-A, PRDX1, PRX1, TDPX2
Gene Symbol
PRDX1
UniProt
Additional Peroxiredoxin 1 Products
Product Documents for Human Peroxiredoxin 1 Antibody
Product Specific Notices for Human Peroxiredoxin 1 Antibody
For research use only
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