Human PBEF/Visfatin Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF4335
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PBEF/Visfatin
Pro27-His491
Accession # P43490
Pro27-His491
Accession # P43490
Specificity
Detects human PBEF/Visfatin in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse PBEF is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human PBEF/Visfatin Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human PBEF/Visfatin
Sample: Recombinant Human PBEF/Visfatin
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PBEF/Visfatin
PBEF (pre-B cell colony-enhancing factor; also visfatin and nicotinamide phosphoribosyltransferase) is a 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the C-terminal 128 amino acids (aa 364‑491). Over aa 27‑491, human PBEF shares 96%, 97%, and 96% aa identity with mouse, porcine and canine PBEF, respectively.
Long Name
Pre-B-Cell Colony-Enhancing Factor 1
Alternate Names
NAmPRTase, NAMPT, NMPRTase, PBEF1, Visfatin
Gene Symbol
NAMPT
UniProt
Additional PBEF/Visfatin Products
Product Documents for Human PBEF/Visfatin Biotinylated Antibody
Product Specific Notices for Human PBEF/Visfatin Biotinylated Antibody
For research use only
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