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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry

Label

Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

E. coli-derived recombinant human Pax6
Met1-Arg272
Accession # P26367

Specificity

Detects human Pax6 in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Pax6 Alexa Fluor® 532-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Pax6

Pax6 (paired box 6; also Oculorhombin) is a 48-50 kDa member of the paired homeobox family of transcription factors.  It is expressed in developing optic vesicle, olfactory dopaminergic neurons, and pancreatic endocrine cells.  Pax6 is a transactivating protein that interacts with MAF, CDX2 and SOX2.  Human Pax6 is 422 amino acids (aa) in length.  It contains an N-terminal paired box DNA-binding domain (aa 4-130), a Gly-rich central region (aa 131-209), a homeodomain (aa 213-269) and a C-terminal Pro/Ser/Thr-rich regulatory domain (aa 279-422).  Phosphorylation of the C-terminal domain at Thr281/304/373 promotes Pax6 activity.  Multiple splice forms of Pax6 exist. There are alternative start sites at Met137 and a position 34 aa upstream of the standard site. There is also a deletion of aa 22-26 and 37-39, plus a 14 aa insertion after Gln47 that generates a C-terminal DNA binding site.  Human and mouse Pax6 are absolutely identical in aa sequence.

Long Name

Paired Box Gene 6

Alternate Names

keratitis), MGC17209, Oculorhombin, paired box 6, paired box protein Pax-6

Entrez Gene IDs

5080 (Human); 18508 (Mouse); 25509 (Rat)

Gene Symbol

PAX6

UniProt

Additional Pax6 Products

Product Documents for Human Pax6 Alexa Fluor® 532-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Pax6 Alexa Fluor® 532-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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