Human Park7/DJ-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3995
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Knockout Validated, Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western
Cited:
ELISA Capture, ELISA Detection
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Park7/DJ-1
Met1-Asp189
Accession # Q99497
Met1-Asp189
Accession # Q99497
Specificity
Detects human Park7/DJ-1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Park7/DJ-1 Antibody
Detection of Human Park7/DJ-1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Park7/DJ-1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Park7/DJ‑1 in HeLa Human Cell Line.
Park7/DJ-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Park7/DJ-1 by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Park7/DJ-1 at approximately 28 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Park7/DJ-1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus)
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus)
Knockout Validated
Park7/DJ‑1
is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in
Park7/DJ‑1 knockout HEK293T cell line.
Simple Western
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line
Western Blot
0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Park7/DJ-1
Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson’s disease.
Long Name
Parkinson Disease 7
Alternate Names
DJ-1, DJ1
Gene Symbol
PARK7
UniProt
Additional Park7/DJ-1 Products
Product Documents for Human Park7/DJ-1 Antibody
Product Specific Notices for Human Park7/DJ-1 Antibody
For research use only
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