Human Orexin B/Hypocretin-2 Antibody

Orexin B in Mouse Hypothalamus.

Orexin B was detected in perfusion fixed frozen sections of mouse brain (hypothalamus) using Mouse Anti-Human Orexin B Monoclonal Antibody (Catalog # MAB734) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the neuronal processes and synaptic boutons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem.

Orexin B, p38a, and Integrin beta1 were detected in perfusion fixed frozen sections of mouse brainstem using Mouse Anti-Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Anti-Mouse Integrin beta1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Porcine Orexin B/Hypocretin-2 by Immunohistochemistry

(A) Cytoplasmatic positivity for orexin B in granulosa cells (20X IHC). Scale bar = 100 µm. (B) Negative control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34204547), licensed under a CC-BY license. Not internally tested by R&D Systems.