Detects human Nectin‑4 in Western blots. In Western blots, approximately 75% cross-reactivity with recombinant mouse Nectin-4 is observed and less than 2% cross-reactivity with recombinant human (rh) Nectin-1, rhNectin-2, and rhNectin-3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Nectin-4 Biotinylated Antibody

Detection of Nectin-4 in MCF-7 cells by Flow Cytometry

MCF-7 cells were stained with Goat Anti-Human Nectin-4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2659, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram) followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Nectin-4 Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

2.5 µg/10⁶ cells
Sample: MCF-7 human breast cancer cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human Nectin-4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-4

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.

Long Name

Poliovirus Receptor Related 4

Alternate Names

LNIR, Nectin4, PRR4, PVRL4

Entrez Gene IDs

81607 (Human); 71740 (Mouse); 498281 (Rat); 102127936 (Cynomolgus Monkey)

Gene Symbol

NECTIN4

UniProt

Q96NY8

Additional Nectin-4 Products

Product Documents for Human Nectin-4 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Nectin-4 Biotinylated Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Nectin-4 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF2659

Key Product Details

Species Reactivity

Validated:

Cited:

Applications