Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human Musashi-1 Monoclonal Antibody (Catalog # MAB2628) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Musashi-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Musashi-1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Musashi-1

Musashi-1 is an RNA-binding protein that is expressed in the developing central nervous system. Expression is highest in proliferating multipotent neural precursor cells and decreases during neuronal differentiation (1, 2).

References

Kaneko, Y. et al. (2000) Dev. Neurosci. 22:139.
Kanemura, Y. et al. (2002) Meth. Mol. Biol. 198:273.

Alternate Names

MSI1, Musashi1

Entrez Gene IDs

4440 (Human)

Gene Symbol

MSI1

Additional Musashi-1 Products

Product Documents for Human Musashi-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Musashi-1 Antibody

For research use only

Citations
Reviews

Human Musashi-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2628

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Musashi-1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Musashi-1 Antibody

Detection of Human Musashi-1 by Western Blot.