Western blot shows lysates of 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse ZIC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5310) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZIC3 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse ZIC3 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 4 using AF5310 in the following applications:

Microarrays (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZIC3

ZIC3 (zinc-finger protein of the cerebellum 3) is a 50 kDa (predicted) member of the GLI C2H2-type Zn-finger protein family. It is expressed in cerebellum, and apparently plays a key role in left-right body axis formation. Human ZIC3 is 467 amino acids (aa) in length. It contains N‑terminal poly-Ala (aa 46‑55) and poly-His (aa 87‑97) segments, followed by five C2H2-type Zn-fingers (aa 253‑410). Within the zinc finger region, there is an overlapping NES (aa 290‑349) and two NLS's (aa 367‑382 and 403‑410). ZIC3 reportedly binds to GLI-3. There is one potential splice form that shows a 49 aa substitution for aa 409‑467, and a second splice form that shows a three aa addition to the C‑terminus. Over aa 380‑467, human ZIC3 displays absolute/100% identity to mouse ZIC3.

Long Name

Zic Family Member 3

Alternate Names

Heterotaxy 1, HTX1, ZNF203

Entrez Gene IDs

7547 (Human); 22773 (Mouse); 367944 (Rat)

Gene Symbol

ZIC3

UniProt

O60481

Additional ZIC3 Products

Product Documents for Human/Mouse ZIC3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse ZIC3 Antibody

For research use only

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