STAT4 immunoprecipitated from lysates of human peripheral blood lymphocytes
Immunoprecipitation
3 µg/500 µg cell lysate
See below
Scientific Data Examples for Human/Mouse STAT4 Antibody
Immunoprecipitation
Immunoprecipitation of Human STAT4.
STAT4 was immunoprecipitated from lysates (1 x 106- 107cells) of human peripheral blood lymphocytes (PBL) following incubation with 3 µg Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4) for 1 hour at 2 - 8 ºC. STAT4-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecipitated STAT4 was detected by Western blot using a 1:2000 dilution of Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4). View our recommended buffer recipes for immunoprecipitation.
Formulation, Preparation and Storage
Formulation
Lyophilized from 0.2 mL antiserum containing 0.02% sodium azide.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Reconstitution Calculator
Background: STAT4
Signal transduction and activator of transcription 4 (STAT4) is a member of the Jak/STAT signal transduction pathway. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 is expressed in subset of tissues including spleen, heart, brain, peripheral blood cells and testis. STAT4 is involved in mediating IL-12 response in lymphocytes and regulating T helper cell differentiation.
Item
Value
Long Name
Signal Transducer and Activator of Transcription 4
Entrez Gene IDs
6775 (Human); 20849 (Mouse)
Alternate Names
SLEB11, STAT4, signal transducer and activator of transcription 4
