Western blot shows lysates of A431 human epithelial carcinoma cell line and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.4 µg/mL of Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TDP-43/TARDBP at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human and Mouse TDP-43/TARDBP by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TDP-43/TARDBP at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

TDP-43/TARDBP in A431 Human Cell Line.

TDP-43/TARDBP was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TDP-43/TARDBP in Human Brain.

TDP-43/TARDBP was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human and Rat TDP-43/TARDBP by Simple Western^TM.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NR8383 rat alveolar macrophage cell line, loaded at 0.2 mg/mL. A specific band was detected for TDP-43/TARDBP at approximately 54 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Detection of Mouse TDP-43/TARDBP by Western Blot

FTLD‐related pathological changes in Tmem106b−/−Grn−/− miceA, BWestern blot analysis of p62, ubiquitin (Ub), TDP‐43 and p‐TDP-43 in RIPA‐ and urea‐soluble fractions from spinal cord (C5–C8) and brain of 5‐month‐old WT, Tmem106b−/−, Grn−/−, and Tmem106b−/−Grn−/− mice. Spinal cord: n = 3; brain: n = 4. Data presented as mean ± SEM. One‐way ANOVA tests with Bonferroni's multiple comparisons: *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001.C, DImmunostaining of p62 and ubiquitin in the spinal cord sections from 5‐month‐old WT, Tmem106b−/−, Grn−/−, and Tmem106b−/−Grn−/− mice. The number of p62 or Ub positive puncta was quantified. n = 3. Data presented as mean ± SEM. One‐way ANOVA tests with Bonferroni's multiple comparisons: ***P < 0.001, ****P < 0.0001. Scale bar = 10 μm.Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32852886), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of TDP-43/TARDBP by Immunoprecipitation.

Immunoprecipitation was performed on cell lysate of HAP1 human near-haploid cell line using 2.0 μg of Mouse Anti-Human TDP-43 Monoclonal Antibody (Catalog # MAB7778) pre-coupled to protein G or protein A beads. Immunoprecipitated TDP-43/TARDBP was detected with a Rabbit Anti-TDP-43 antibody. The Ponceau stained transfers of each blot are shown. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitated. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).

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Applications for Human/Mouse/Rat TDP-43/TARDBP Antibody

Application

Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line

Immunohistochemistry

1-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Immunoprecipitation

2 µg/1 mg cell lysate
Sample: Cell lysate of HAP1 human near-haploid cell line

Simple Western

5 µg/mL
Sample: A431 human epithelial carcinoma cell line and NR8383 rat alveolar macrophage cell line

Western Blot

0.4-1 µg/mL
Sample: A431 human epithelial carcinoma cell line, NR8383 rat alveolar macrophage cell line, HeLa human cervical epithelial carcinoma cell line, and RAW 264.7 mouse monocyte/macrophage cell line

Reviewed Applications

Read 2 reviews rated 4.5 using MAB7778 in the following applications:

Immunohistochemistry (1 Review)
Immunohistochemistry-Paraffin (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TDP-43/TARDBP

TDP-43 (TAR DNA binding protein of 43 kDa; gene name TARDBP) is a 414 amino acid (aa) nuclear RNA/DNA binding phosphoprotein that regulates transcription and splicing. TDP-43 is aberrantly expressed in most forms of amylotrophic lateral sclerosis and frontotemporal lobar degeneration. In these cases it is absent from the nucleus and accumulates, along with a truncated 20-28 kDa form, in ubiquitin-containing inclusion bodies. TDP-43 can also influence CFTR exon 9 skipping in cystic fibrosis. Within aa 1-103, human and mouse TDP-43 share 100% aa sequence identity, and 99% aa identity with rat TDP-43.

Long Name

TAR DNA-binding Protein 43

Alternate Names

ALS10, TARDBP, TDP43

Entrez Gene IDs

23435 (Human)

Gene Symbol

TARDBP

UniProt

Q13148

Additional TDP-43/TARDBP Products

Product Documents for Human/Mouse/Rat TDP-43/TARDBP Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse/Rat TDP-43/TARDBP Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat TDP-43/TARDBP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7778

Key Product Details

Species Reactivity

Validated:

Cited:

Applications