Human/Mouse/Rat RSK Pan Specific Antibody

Detection of Human, Mouse, and Rat RSK by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat RSK Pan Specific Monoclonal Antibody (Catalog # MAB2056) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for RSK at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human RSK by Simple Western^TM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for RSK at approximately 86 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat RSK Pan Specific Monoclonal Antibody (Catalog # MAB2056). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of RSK Pan Specific by Western Blot

MEK1 mutations at the identified regions had different sensitivities to MEK and BRAF inhibitors in cell viability and MAPK pathway signalling assays.a Dose response curves of A375 cell line viability in response to selumetinib, trametinib or vemurafenib. The upper panel of curves include the MEK1 activating mutations and the lower panel include the allosteric pocket influencing mutations. Cell viability is shown as percentage to the DMSO control. Data points with three replicates are shown. b MAPK pathway signalling upon selumetinib treatment. Effects of 2 h treatment with 0.1 µM or 10 µM selumetinib on MAPK pathway signalling were tested in the A375 cell lines. c MAPK pathway signalling upon vemurafenib treatment. Effects of 2 h treatment with 0.3 µM or 10 µM vemurafenib on MAPK pathway signalling were tested in the A375 cell lines. DMSO treatment is indicated as ‘-’. Band intensity was quantitated by densitometry. And fold changes of pMEK (S218/S222)/tMEK ratios to the wild-type are indicated below the plots as numbers. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s42003-025-07966-4), licensed under a CC-BY license. Not internally tested by R&D Systems.