Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Alexa Fluor® 700-conjugated Antibody

Name: Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Alexa Fluor® 700-conjugated Antibody
Brand: R&D Systems
SKU: AF887N

R&D Systems, part of Bio-Techne | Catalog # AF887N

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Product Datasheet / COA

Key Product Details

Species Reactivity:

Human, Mouse, Rat

Immunogen:

Phosphopeptide containing human, mouse, and rat Akt1 S473 site

Applications:

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot

Label:

Alexa Fluor 700 (Excitation=675-700 nm, Emission=723 nm)

Specificity:

Detects human, mouse and rat Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively.

View Full Technical Details for Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Alexa Fluor® 700-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.
CyTOF-ready	Optimal dilution of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen and protein A Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Akt

Akt1, Akt2, and Akt3 make up a family of serine/threonine kinases, often activated downstream of growth factor receptors and PI 3-Kinase. Each Akt family member contains an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain. They have putative roles in cell proliferation and survival, and Akt perturbations are thought to play an important role in tumorigenesis.

Item	Value
Long Name	v-Akt Murine Thymoma Viral Oncogene Homolog
Alternate Names	RAC-ALPHA, AKT serine/threonine kinase 1, Akt, PKB, PKB-ALPHA, PRKBA, RAC

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