Human/Mouse/Rat PGK1 Alexa Fluor™ Plus 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5455AFP647
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PGK1
Specificity
Detects endogenous human, mouse and rat PGK1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: PGK1
Phosphoglycerate kinase-1 (PGK1) is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3 phosphoglycerate. The gene encoding PGK1 is X-linked. Mutations of this gene may cause phosphoglycerate kinase deficiency, which is characterized by hemolytic anemia, muscle stiffness and mental retardation (1‑3). PGK1 is induced by oxidative stress through the induction of hypoxia-inducible factor 1 alpha and is a potential biomarker and therapeutic target for cancer (4‑7).
References
- Beutler, E. (2002) Br. J. Haematol. 136:3.
- Flanagan, J.M. et al. (2006) Br. J. Haematol. 134:233.
- Svaasand, E.K. et al. (2007) Muscle Nerve. 36:579.
- Hwang, T.L. et al. (2006) Proteomics. 6:2259.
- Wang, J. et al. (2007) Cancer Res. 67:149.
- Zieker, D. et al. (2008) Cell Physiol. Biochem. 21:429.
- Jang, C.H. et al. (2008) Biosci. Biotechnol. Biochem. 72:1799.
Long Name
Phosphoglycerate Kinase-1
Alternate Names
MIG10, PGKA, PRP2
Gene Symbol
PGK1
UniProt
Additional PGK1 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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