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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human LHX5
Leu265-Trp402
Accession # Q9H2C1

Specificity

Detects recombinant human LHX5 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) LIM1 is observed, and less than 5% cross-reactivity with rhLHX2, and rhLHX9 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human/Mouse/Rat LHX5 Alexa Fluor® 750-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: LHX5

LHX5 (LIM/homeobox protein 5) is a member of the LIM homeobox gene family. It is expressed in embryonic CNS, and appears to contribute to the formation and maintenance of multiple neuronal phenotypes. In the cerebellum, LHX5 promotes Purkinje cell development; in the spinal cord, LHX5 maintains a GABAergic phenotype in select dorsal horn interneurons. LHX5 is reported to act as a Wnt pathway antagonist through its stimulation of sFRP-1 and -5 secretion. Human LHX5 is 402 amino acids (aa) in length. It contains two LIM Zn-binding domains (aa 3-61 and 62-125), one phosphorylation site at Tyr119, and a DNA-binding homeodomain (aa 180‑239). Over aa 265-402, human LHX5 shares 97% aa identity with mouse LHX5, and 60% aa identity with its paralogue (common ancestoral) gene, LHX1.

Long Name

LIM (Lin11:Isl1:Mec3)/Homeobox Protein 5

Alternate Names

LIM homeobox 5, LIM homeobox protein 5, LIM/homeobox protein Lhx5, MGC129689

Entrez Gene IDs

64211 (Human); 16873 (Mouse); 124451 (Rat)

Gene Symbol

LHX5

UniProt

Additional LHX5 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat LHX5 Alexa Fluor® 750-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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