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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Western Blot

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 845208

Product Specifications

Immunogen

E. coli-derived recombinant human Importin β/KPNB1
Met1-Gly155
Accession # Q14974

Specificity

Detects human Importin beta/KPNB1 in ELISA and Western Blot. It detects mouse and rat Importin beta/KPNB1 in Western Blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Importin beta/KPNB1

KPNB1 (Karyopherin subunit beta 1), also called Importin beta1, NTF97 (nuclear transport factor, 97 kDa) or PTAC97 (pore targeting complex, 97 kDa), is a member of the Importin b family of proteins. It is ubiquitously expressed and plays many roles in the nucleus. Primarily, KPNB1 transports various complexes from cytoplasm into nucleus, either alone or with a KPNA adaptor. The 876 amino acid (aa) human KPNB1 contains an N-terminal IBB/Importin b domain (aa 21-101), and eight HEAT (solenoid) domains (aa 124-726). A 731 aa splice variant begins with Met146, while an 845 aa variant shows a 2 aa substitution for aa 702-734. Binding sites for adaptors and for its regulator, Ran-GTP, overlap within the HEAT domains. Human KPNB1 aa 1-155 shares 99% and 97% aa sequence identity with mouse and rat KPNB1, respectively.

Long Name

Karyopherin beta 1

Alternate Names

IMB1, Impnb, Importin 1, IPOB, KPNB1, NTF97, PTAC97

Entrez Gene IDs

3837 (Human); 16211 (Mouse); 24917 (Rat)

Gene Symbol

KPNB1

UniProt

Additional Importin beta/KPNB1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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