Species Reactivity:

Human, Mouse, Rat

Immunogen:

E. coli-derived recombinant human GAPDH/G3PDH
Met1-Ala150
Accession # P04406

Applications:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label:

Alexa Fluor 488 (Excitation=488 nm, Emission=515-545 nm)

Specificity:

Detects human, mouse, and rat GAPDH/G3PDH in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human GAPDH-2 is observed.

View Full Technical Details for Human/Mouse/Rat GAPDH Alexa Fluor® 488-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.
Immunocytochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: GAPDH

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a 36‑40 kDa member of the GAPDH family of enzymes. It is a widely expressed heterotetramer that is found in both the nucleus and cytoplasm. Although GAPDH was initially identified as a glycolytic enzyme that converted G3P into 1,3 diphosphoglycerate, it is now recognized to participate in no less than endocytosis, membrane fusion, vesicular secretory transport, DNA replication and repair, and apoptosis. Human GAPDH is 335 amino acids (aa) in length and contains two NAD binding sites (Asp35 and Asn316) with a catalytic region between aa 151‑155. GAPDH contains more than 19 posttranslational modifications, including methylation, deamidation and phosphorylation. One splice variant shows a 10 aa substitution for aa 319‑335. Over amino acid 1‑150, human GAPDH shares 92% aa identity with mouse GAPDH.

Item	Value
Long Name	Glyceraldehyde-3-phosphate Dehydrogenase
Entrez Gene IDs	2597 (Human); 14433 (Mouse); 24383 (Rat)
Alternate Names	EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, G3PDH, GAPD, GAPDH, MGC88685, Peptidyl-cysteine S-nitrosylase GAPDH, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase

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Human/Mouse/Rat GAPDH Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5718G

Key Product Details