eIF5A (Elongation initiation factor 5A; also eIF5A1, Rev-binding factor and eIF-4D) is a 16-19 kDa member of the eIF5A family of molecules. It is ubiquitously expressed, being found in all human cells examined. eIF5A acts as a stabilizer of RNA transcripts and acts as a positive modulator of p53-mediated apoptosis. It also may be involved in the formation of the first peptide bond during select protein synthesis, and is known to be essential for cell cycle progression through G1/S. The human eIF5A precursor is 154 amino acids (aa) in length. It contains a 71 aa DOHH (deoxyhypusine hydroxylase)-binding site (aa 20-90) and a 69 aa RNA-binding domain (aa 84-152). eIF5A undergoes a posttranslational modification that converts the precursor peptide into a mature molecule. This modification occurs on Lys50, and involves the transfer and hydroxylation of an aminobutyl sequence from spermidine, creating a unique amino acid termed hypusine (hydroxyputrisine lysine). When hypusine is unacetylated, eIF5A is active; when acetylated, eIF5A is inactive. Nonhypusinated eIF5A is found in both cytoplasm and nucleus, while hypusinated eIF5A is targeted to the cytoplasm. There is one isoform variant that shows a 32 aa substitution for aa 1-32. Over aa 85-154, human and mouse are identical in aa sequence. Over the same aa sequence, human eIF5A (on Chr 17p13) also shares 98% and 69% aa identity with human eIF5A1-Like (on Chr 10q22) and human eiF5A2 (on Chr 3q26), respectively.

Long Name

Eukaryotic Translation Initiation Factor 5A

Alternate Names

eIF4D, Rev-binding Factor

Entrez Gene IDs

1984 (Human); 276770 (Mouse); 287444 (Rat)

Gene Symbol

EIF5A

UniProt

P63241 (Human)

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Product Specific Notices for Human/Mouse/Rat eIF5A Alexa Fluor® 350-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Human/Mouse/Rat eIF5A Alexa Fluor® 350-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7558U

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Background: eIF5A