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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 652308

Product Specifications

Immunogen

E. coli-derived recombinant human DGK-θ
Asp696-Lys839
Accession # P52824

Specificity

Detects DGK-theta in direct ELISAs and Western blots. In direct ELISAs and Western blolts, no cross-reactivity with recombinant human DGK-alpha, -beta, -gamma, -epsilon, -iota, -kappa, or - zeta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DGK-theta

Diacylglycerol kinase theta (DGK-theta) is an approximately 110 kDa kinase that phosphorylates the lipid diacylglycerol. DGK-theta binds to RhoA, Akt, PKC epsilon and eta, and Steroidogenic Factor. It translocates to the nucleus or to the plasma membrane in response to NGF, TPA, or GPCR stimulation (thrombin, bradykinin, norepinephrine). DGK-theta contains three Cys-rich zinc finger regions (aa 60-108, aa 121-168, and aa 183-234), a Ras-associating domain (aa 395-494), two LxxLL motifs (aa 555-559 and aa 574-578), and a catalytic domain (aa 590-714). Over aa 696-839, human DGK-theta shares 90% aa sequence identity with mouse and rat DGK-theta.

Long Name

Diacylglycerol Kinase, theta 110kDa

Alternate Names

DGKQ, DGKtheta

Entrez Gene IDs

1609 (Human)

Gene Symbol

DGKQ

UniProt

Additional DGK-theta Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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