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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 2.7D5

Product Specifications

Immunogen

Pigeon Cytochrome c

Specificity

Detects native human, mouse, and rat Cytochrome c.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications

Application
Recommended Usage

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Cytochrome c

Cytochrome c is a critical mitochondrial outer membrane-associated protein in the electron transport chain (1). Reduction and oxidation of an iron molecule (Fe3+ to Fe2+ and back) within its central heme group allow it to receive an electron from the cytochrome c1 subunit of cytochrome reductase and pass it to cytochrome a within the cytochrome oxidase complex (1). Release of cytochrome c from mitochondria occurs during initiation of apoptotic pathways, either by mechanisms that rupture or that do not rupture the mitochondial membrane (2, 3). Once in the cytosol, cytochrome c forms a complex with procaspase 9, recruiting Apaf-1 (apoptotic protease activating factor-1) and dATP to form apoptosomes, which activate caspase and ultimately destroy the cell (2, 3). Cytochrome c is highly conserved, with 90% or greater amino acid identity among human, mouse, rat, cow and dog sequences (4). After cytochrome c translation, the initial methionine is cleaved to form the 104 amino acid mature form.

Alternate Names

CYCS

Entrez Gene IDs

54205 (Human)

Gene Symbol

CYCS

Additional Cytochrome c Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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