Recognizes the A2B5 cell surface ganglioside epitope found on type II astrocytes, cells involved in gliogenesis, and cells committed to the oligodendrocyte lineage (1‑4).

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human/Mouse/Rat/Chicken A2B5 Antibody

Detection of A2B5 in NTera-2 Human Cell Line by Flow Cytometry.

NTera-2 human testicular embryonic carcinoma cell line either treated with 10 µM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Monoclonal Antibody (Catalog # MAB1416), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). View our protocol for Staining Membrane-associated Proteins.

A2B5 in NTera-2 Human Cell Line.

A2B5 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line (differentiated with retinoic acid for 10 days) using Mouse Anti-Human/Mouse/Rat/ Chicken A2B5 Monoclonal Antibody (Catalog # MAB1416) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; Catalog # NL019) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat/Chicken A2B5 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: NTera‑2 human testicular embryonic carcinoma cell line treated with retinoic acid

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line (differentiated with retinoic acid for 10 days)

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: A2B5

A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells (1).

References

Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913.
Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51.
Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.

Alternate Names

A2B5 marker

Additional A2B5 Products

Product Documents for Human/Mouse/Rat/Chicken A2B5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat/Chicken A2B5 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human/Mouse/Rat/Chicken A2B5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1416

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications