Human/Mouse/Rat Caveolin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5736
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Western Blot, Immunocytochemistry
Cited:
Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 7C8
Product Specifications
Immunogen
Puified rat adipocyte low density microsomes
Accession # P41350
Accession # P41350
Specificity
Detects endogenous human, mouse and rat Caveolin-1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat Caveolin-1 Antibody
Detection of Human Caveolin-1 by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells and HMVEC human microvascular endothelial cells. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Caveolin-1 Monoclonal Antibody (Catalog # MAB5736) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caveolin-1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Caveolin‑1 in A431 Human Cell Line.
Caveolin-1 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Caveolin-1 Monoclonal Antibody (Catalog # MAB5736) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat Caveolin-1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells and HMVEC human microvascular endothelial cells
Sample: HUVEC human umbilical vein endothelial cells and HMVEC human microvascular endothelial cells
Reviewed Applications
Read 2 reviews rated 5 using MAB5736 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caveolin-1
Alternate Names
CAV1, Caveolin1, MSTP085, VIP21
Gene Symbol
CAV1
UniProt
Additional Caveolin-1 Products
Product Documents for Human/Mouse/Rat Caveolin-1 Antibody
Product Specific Notices for Human/Mouse/Rat Caveolin-1 Antibody
For research use only
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