Human/Mouse Orexin R2/HCRTR2 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC5246A
Discontinued Product
IC5246A has been discontinued.
An alternative/replacement product is available:
IC5246R. View all Orexin R2/HCRTR2 products.
Key Product Details
Species Reactivity
Human, Mouse
Applications
Intracellular Staining by Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 456738
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human Orexin R2/HCRTR2
Met1-Trp444
Accession # AAC39602
Met1-Trp444
Accession # AAC39602
Specificity
Detects human and mouse Orexin R2/HCRTR2. Stains human and mouse Orexin R2/HCRTR2 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse Orexin R2/HCRTR2 APC-conjugated Antibody
Detection of Orexin R2/HCRTR2 in A172 Human Cell Line by Flow Cytometry.
A172 human glioblastoma cell line was stained with Mouse Anti-Human/Mouse Orexin R2/HCRTR2 APC-conjugated Monoclonal Antibody (Catalog # IC5246A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of Orexin R2/HCRTR2 in Neuro‑2A Mouse Cell Line by Flow Cytometry.
Neuro-2A mouse neuroblastoma cell line was stained with Mouse Anti-Human/Mouse Orexin R2/HCRTR2 APC-conjugated Monoclonal Antibody (Catalog # IC5246A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse Orexin R2/HCRTR2 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: A172 human glioblastoma cell line and Neuro‑2A mouse neuroblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: A172 human glioblastoma cell line and Neuro‑2A mouse neuroblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Orexin R2/HCRTR2
Hypocretin Receptor 2 (HCRTR2), also known as Orexin Receptor 2 or OX2R, is a 40 kDa 7-transmembrane G-protein-coupled glycoprotein that is a high affinity receptor for Orexins A and B (Hypocretins 1 and 2). In mouse brain, engagement of HCRTRs promotes wakefulness, such that absence of either orexins or their receptors creates a narcolepsy-like state. It also influences reward circuits involving food or addictive drugs. The extracellular portions of human HCRTR2 share 92% and 93% aa identity with corresponding portions of mouse and rat HCRTR2, respectively.
Long Name
Hypocretin Receptor Type 2
Alternate Names
HCRTR2, Orexin Receptor 2, OrexinR2, OX2R
Gene Symbol
HCRTR2
UniProt
Additional Orexin R2/HCRTR2 Products
Product Documents for Human/Mouse Orexin R2/HCRTR2 APC-conjugated Antibody
Product Specific Notices for Human/Mouse Orexin R2/HCRTR2 APC-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...