Human/Mouse Oct-3/4 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC1759A
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Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 240408
Product Specifications
Immunogen
E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860
Met1-Asn265 (Met262Leu)
Accession # Q01860
Specificity
Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human/Mouse Oct-3/4 APC-conjugated Antibody
Detection of Oct-3/4 in NTERA-2 cells by Flow Cytometry
NTERA-2 cells were stained with Rat Anti-Human/Mouse Oct-3/4 APC-conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Detection of Oct-3/4 in BG01V Human Cells by Flow Cytometry.
BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 APC-conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
Detection of Oct-3/4 in iPSC cells by Flow Cytometry.
iPSC cells were stained with Rat Anti-Human/Mouse Oct-3/4 APC-conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse Oct-3/4 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: NTERA-2 human testicular embryonic carcinoma cell line or BG01V human embryonic stem cells and D3 mouse embryonic stem cell line treated with Retinoic Acid were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) and iPSC cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Sample: NTERA-2 human testicular embryonic carcinoma cell line or BG01V human embryonic stem cells and D3 mouse embryonic stem cell line treated with Retinoic Acid were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) and iPSC cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Oct-3/4
Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.
References
-
Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
-
Scholer, H.R. et al. (1989) EMBO J. 8:2543.
-
Rosner, M.H. et al. (1990) Nature 345:686.
- Niwa, H. et al. (2000) Nat. Genet. 24:372.
Long Name
Octamer-binding Protein 3/4
Alternate Names
3-Oct, Oct4, Otf3g, Otf4, Pou5f1
Gene Symbol
POU5F1
UniProt
Additional Oct-3/4 Products
Product Documents for Human/Mouse Oct-3/4 APC-conjugated Antibody
Product Specific Notices for Human/Mouse Oct-3/4 APC-conjugated Antibody
For research use only
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