Human/Mouse Nrf2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3925
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Nrf2
Met17-Asn605
Accession # Q16236
Met17-Asn605
Accession # Q16236
Specificity
Detects human Nrf2 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse Nrf2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse Nrf2 Antibody
Detection of Human Nrf2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 10 µM MG 132 (Catalog # 1748) for 4 hours. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Nrf2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3925) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Nrf2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Nrf2 by Western Blot.
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse Nrf2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3925) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Nrf2 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Nrf2 by Western Blot
Effect of RSO on Nrf2-Keap1 pathway of LPS-induced RAW 264.7 cells. Relative mRNA expression of Nrf2 (A), HO-1 (B), NQO1 (C), and Keap1 (D) genes normalized to beta-actin and expressed as fold change of the control group, n = 3. (E) The protein levels of Nrf2 and Keap1 were analyzed by immunoblotting with beta-actin as the internal reference protein for normalization, n = 3. Relative protein expression of Nrf2 (F) and Keap1 (G) were expressed as fold change of the LPS-induced group. ** p < 0.01 vs control group; # p < 0.05, ## p < 0.01 vs LPS-induced group. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2072-6643/14/7/1349), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human/Mouse Nrf2 Antibody
Application
Recommended Usage
Western Blot
0.2-2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line treated with MG 132 (Catalog # 1748) and RAW 264.7 mouse monocyte/macrophage cell line treated with LPS
Sample: HepG2 human hepatocellular carcinoma cell line treated with MG 132 (Catalog # 1748) and RAW 264.7 mouse monocyte/macrophage cell line treated with LPS
Reviewed Applications
Read 1 review rated 5 using AF3925 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nrf2
NF-E2 related factor 2 (Nrf2) is a member of the cap ‘n’ collar family of basic leucine zipper transcription factors. By binding antioxidant response elements (AREs), Nrf2 activates the transcription of genes encoding antioxidant and phase II drug-metabolizing enzymes needed to protect cells against oxidative damage. Transcriptional targets regulated by Nrf2 include genes encoding glutathione S-transferases, superoxide dismutase-1, and heme oxygenase-1.
Long Name
NF-E2-related Factor 2
Alternate Names
NFE2L2
Gene Symbol
NFE2L2
UniProt
Additional Nrf2 Products
Product Documents for Human/Mouse Nrf2 Antibody
Product Specific Notices for Human/Mouse Nrf2 Antibody
For research use only
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