Human/Mouse MCPIP1 Antibody

Detection of Human and Mouse MCPIP1 by Western Blot.

Western blot shows lysates of untreated (-) HeLa human cervical epithelial carcinoma cell line and RAW 264.7 mouse monocyte/macrophage cell line and THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 1 µg/mL LPS for 24 hours or 10 µg/mL LPS for 3 hours, respectively. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MCPIP1 Monoclonal Antibody (Catalog # MAB7875) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MCPIP1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse MCPIP1 by Western Blot

Effects of single or combined TRAF6 KO and MALT1 paracaspase mutation on signaling in CD4+ T cells. (A) Schematic overview of the four conditional mouse strains used in the analyses. (B) Western blots showing MALT1 substrate cleavage in unstimulated purified CD4+ T cells from Wthet, T6-delta T;M1 PD-T, Traf6-delta T and Malt1 PD-T mice (two independent mice each). Asterisks indicate unspecific signals. (C) Analyses of NF-kappa B activation (EMSA), p65 phosphorylation and MALT1 substrate cleavage (Western blot, WB) in PMA/Ionomycin (P/I) stimulated purified CD4+ T cells of mice as depicted in (A). (D) Expression of ICOS on CD4+ and CD8+ T cells by flow cytometric analysis of spleen of mice as depicted in (A). Bars show the means ± SEM, and P values were calculated by one-way ANOVA with Tukey’s multiple comparison test. All analyses were performed with mice 9-11 weeks of age. Each dot represents one mouse. Ct, C-terminus; l.e., long exposure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36761777), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse MCPIP1 by Western Blot

Effects of single or combined TRAF6 KO and MALT1 paracaspase mutation on signaling in CD4+ T cells. (A) Schematic overview of the four conditional mouse strains used in the analyses. (B) Western blots showing MALT1 substrate cleavage in unstimulated purified CD4+ T cells from Wthet, T6-delta T;M1 PD-T, Traf6-delta T and Malt1 PD-T mice (two independent mice each). Asterisks indicate unspecific signals. (C) Analyses of NF-kappa B activation (EMSA), p65 phosphorylation and MALT1 substrate cleavage (Western blot, WB) in PMA/Ionomycin (P/I) stimulated purified CD4+ T cells of mice as depicted in (A). (D) Expression of ICOS on CD4+ and CD8+ T cells by flow cytometric analysis of spleen of mice as depicted in (A). Bars show the means ± SEM, and P values were calculated by one-way ANOVA with Tukey’s multiple comparison test. All analyses were performed with mice 9-11 weeks of age. Each dot represents one mouse. Ct, C-terminus; l.e., long exposure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36761777), licensed under a CC-BY license. Not internally tested by R&D Systems.