Western blot shows lysates of human colon tissue and human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse Lumican Monoclonal Antibody (Catalog # MAB2846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Lumican at approximately 60-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse Lumican Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: Human colon tissue and human heart tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lumican

Lumican is a secreted matrix protein that belongs to the small leucine-rich proteoglycan (SLRP) class II family. Mature Lumican has a negatively charged N-terminal domain that contains tyrosine sulfates, followed by twelve leucine-rich repeats, which are flanked by cysteine loops. Lumican is expressed widely in mammalian connective tissues, while corneal Lumican exists as a keratan sulfate proteoglycan in adult cartilage, Lumican exists primarily as a glycoprotein and lacks keratan sulfate. The amino acid sequence of human Lumican shares 88% amino acid sequence identity with that of mature mouse Lumican.

Alternate Names

LDC, LUM, SLRR2D

Entrez Gene IDs

4060 (Human); 17022 (Mouse)

Gene Symbol

LUM

UniProt

P51884

Additional Lumican Products

Product Documents for Human/Mouse Lumican Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Lumican Antibody

For research use only

Citations
Reviews

Human/Mouse Lumican Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2846

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Lumican Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse Lumican Antibody

Detection of Human Lumican by Western Blot.