Human/Mouse Lefty Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF746
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Lefty-1
Leu136-Pro368
Accession # Q64280.1
Leu136-Pro368
Accession # Q64280.1
Specificity
Detects human and mouse Lefty in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human/Mouse Lefty Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Lefty-A (Catalog # 746-LF)
Recombinant Mouse Lefty-1 (Catalog # 994-LF)
Sample: Recombinant Human Lefty-A (Catalog # 746-LF)
Recombinant Mouse Lefty-1 (Catalog # 994-LF)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lefty
Lefty proteins are novel TGF-beta ligands that function as antagonists of Nodal signaling. The expression of Lefty proteins on the left side of the developing mouse embryo earned this protein family its name. Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A and Lefty-B). By amino acid sequence, human Lefty-A and Lefty-B are more similar to each other (96%) than to either Lefty-1 or Lefty-2 in the mouse (81-82%). The Leftys contains some of the features of the cysteine-knot structure conserved among TGF-beta-related proteins, but also some structural differences. Specifically, they lack the alpha-helical segment important for ligand dimerization as well as the cysteine residue involved in stabilization of dimers.
Alternate Names
AI450052;Ebaf;LEFTB;Lefty;LEFTY1;lefty-1;LEFTYB;RGD1561867;Stra3;Tgfb4;UNQ278/PRO317
Gene Symbol
LEFTY2
UniProt
Additional Lefty Products
Product Documents for Human/Mouse Lefty Biotinylated Antibody
Product Specific Notices for Human/Mouse Lefty Biotinylated Antibody
For research use only
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