Human/Mouse Kilon/NEGR1 Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5394N
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kilon/NEGR1
Val38-Gly324
Accession # Q7Z3B1
Val38-Gly324
Accession # Q7Z3B1
Specificity
Detects Kilon/NEGR1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) LAMP and rhNCAM-L1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Kilon/NEGR1
Kindred of IgLON (Kilon; also neuronal growth regulator 1 (NEGR1) and neurotractin) is a 46 kDa member of the IgLON family of molecules. This cell adhesion family includes the proteins LAMP, OBCAM, neurotrimin, CEPU-1, AvGP50, and GP55 (1). Human Kilon is synthesized as a 354 amino acid (aa) precursor that contains a 37 aa signal sequence, a 287 aa mature chain, and a 30 aa propeptide. The mature chain consists of three C2 Ig-like domains, six potential sites for N-linked glycosylation, and a GPI anchor. In addition, there are three sets of cysteines that have the potential to form intradomain disulfide linkages in each of the mature chain's Ig-like domains (1). Human Kilon shares 97% aa sequence identity with mouse and rat Kilon. Expression of Kilon is restricted to the brain, specifically in the cerebrum, brain stem, and hippocampus, with much less expression in the cerebellum (1). In the rat, it was shown that Kilon is already expressed in the E16 stage, and its level gradually increases during development (1). In the cerebral cortex, numerous puncta of Kilon immunoreactivity were visible in all regions, and most were densely distributed in large neurons of layer V (1). These neurons were identified as pyramidal neurons because of their soma location in layer V, large soma size, and extension of their apical dendrite to layer I (1). Kilon may be involved in cell-adhesion and may function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain (1, 2).
Long Name
A Kindred of IgLON /Neuronal Growth Regulator 1
Alternate Names
NEGR1, Neurotractin, Ntra
Gene Symbol
NEGR1
UniProt
Additional Kilon/NEGR1 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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