Human/Mouse HO-1/HMOX1/HSP32 Antibody

Detection of Human/Mouse HO-1/HMOX1/HSP32 by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HO-1/HMOX1/HSP32 Monoclonal Antibody (Catalog # MAB3776) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western Blot Shows Human HO‑1/HMOX1/HSP32 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HO-1/HMOX1/HSP32 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse HO-1/HMOX1/HSP32 Monoclonal Antibody (Catalog # MAB3776) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of HO-1/HMOX1/HSP32 by Immunohistochemistry

beta2-AR and EGFR pathways regulate the ROS metabolism NRF-2 mediated. Evaluation of the ROS metabolism in cancer cells directly obtained from the HNSCC-bearing mice. a Western Blot analysis of Nrf-2 expression in both cytoplasmic and nuclear protein extracts. ICI treatment reduced the Nrf-2 nuclear translocation, in a more evident way if in combination with CTX. b RT-PCR for the HO-1, NQO-1, GCLC, and G6PD gene expression level analysis. The beta2-AR blockade diminishes the gene expression level of HO-1, with a synergistic effect in combination with CTX, which is able to induce a milder effect by itself. The MEK 1/2 inhibition did not replicate this effect. No significant effects have been observed about the NQO-1 expression. The expression of GCLC and G6PDH is significantly reduced only after treatment with ICI in combination with CTX. c Immunohistochemistry assay on mice tongues engrafted with UMSCC 103 for the HO-1 and NQO1 detection. The ICI and CTX treatments sensibly reduced the expression of HO1, which increases if we combine the drugs. The expression of NQO1 was affected only in mice subjected to the combination of ICI plus CTX. d Cell-ROX assay for the evaluation of the oxidative stress in UMSCC 103 induced by our drugs. With both flow cytometer and e fluorescent microscopy analysis, we observed an increased level of oxidation after treatment with the beta2-AR inhibitor, as well as with CTX and U0126. The same drugs are even more effective in combination, but this increased effect is counteracted by the KI696. (*P ≤ 0.05; **P ≤ 0.01; ****P ≤ 0.0001 vs. CTR). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37723219), licensed under a CC-BY license. Not internally tested by R&D Systems.