Detects human and mouse Glypican 5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant mouse Glypican 5 is observed and no cross‑reactivity with recombinant human Glypican 2, 3 or 6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human/Mouse Glypican 5 Antibody

Detection of Glypican 5 by Immunocytochemistry/ Immunofluorescence

Localization of GPC5 and Rab11 in U3DT cells treated with RAB11A-siRNA.Immunofluorescence images of cells were obtained using a Leica SP-8 confocal microscope, and the pixel sum was estimated with the SP8 software (RAS X). Immunofluorescence image (G) of trypsinized cells were obtained using a Leica SP-8 immunofluorescence microscope. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33606708), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse Glypican 5 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: H9 human cutaneous T lymphoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human Glypican 5 (Catalog # 2607-G5)
Recombinant Mouse Glypican 5 (Catalog # 2689-G5)

Reviewed Applications

Read 1 review rated 4 using MAB2607 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 5

Glypican 5 (GPC5) belongs to the Glypican family of heparan sulfate proteoglycans that are linked to the cell surface through a glycosyl-phosphatidylinositol anchor. Glypican 5 is expressed primarily in embryonic neurons and mesenchyme.

Alternate Names

GPC5

Entrez Gene IDs

2262 (Human); 103978 (Mouse)

Gene Symbol

GPC5

UniProt

P78333

Additional Glypican 5 Products

Product Documents for Human/Mouse Glypican 5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Glypican 5 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human/Mouse Glypican 5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2607

Key Product Details

Species Reactivity

Validated:

Cited:

Applications