Human/Mouse Glucagon Antibody

Glucagon in beta TC-6 Mouse Cell Line.

Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Glucagon in betaTC‑6 Mouse Cell Line by Flow Cytometry.

betaTC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Glucagon by Immunohistochemistry

LRRK2 G2019S mice exhibit altered metabolic profile and impaired insulin secretion. A-C Quantitative analysis of A body weight (gr), B blood glucose (mg/dL), and C circulating insulin (µg/L) in LRRK2 WT and G2019S mice (n = 13 WT and 14 G2019S mice). Blood glucose and insulin levels were measured under fasting conditions (6 h, light cycle). Data are reported as mean ± SD. Unpaired Student’s T-test: *p < 0.05; ** p < 0.01. D Representative immunofluorescence images of islets of Langerhans from LRRK2 WT and G2019S mice stained with anti-insulin (red), anti-glucagon (green), and anti-somatostatin (blue) antibodies. Scale bar: 40 μm. E Size area (µm2) of islets of Langerhans from LRRK2 WT and G2019S mice (n = 5 mice/group). Data are reported as mean ± SD. Unpaired Student’s T-test: *p < 0.05. F Glucose-stimulated (20 mM) insulin secretion in islets of Langerhans isolated from LRRK2 WT and G2019S mice (n = 3 independent experiments in triplicate). Data are reported as mean ± SD. Two-way ANOVA: *p < 0.05, **p < 0.01. G Stimulatory index of insulin secretion. Data (mean ± SD) are expressed as stimulated over basal release (n = 3 independent experiments in triplicate). Unpaired Student’s T-test: * p < 0.05 Image collected and cropped by CiteAb from the following open publication (https://link.springer.com/10.1007/s00018-025-05810-w), licensed under a CC-BY license. Not internally tested by R&D Systems.